Accurate & Sensitive
Optima 96’s uses 6 separate thermoelectric modules resulting in high level of accuracy and sensitivity in detecting and amplifying target DNA sequences, even in low concentrations.
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Gradient PCR - Optima96
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Product Information
Product code : PR122
Representing the forefront of PCR technology, the Gradient PCR Optima 96 features six separate imported thermo-electric modules for precise temperature control and gradient PCR to ensure performance on the edges of 96 well plates. The device is easy to use with a high resolution, 7-inch LCD touchscreen panel, and has many advanced features such as touchdown (TD) PCR mode and long-range PCR modes. The Optima 96 also boasts one of the fastest heating/cooling rates in the industry for better nucleotide pairing performance. Designed to be compatible with 0.2ml PCR tubes & strips, and 0.2ml 96-well plates (non-skirted and semi-skirted), this device is an excellent choice for researchers seeking precise temperature control and advanced features for PCR optimization and performance.
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How it works
Prep & Load PCR reaction mixture
Include template DNA, primers, nucleotides, and Taq polymerase and load reaction mixture into PCR tube. Select a prebuilt program, modify program template, or build a new thermocycling program from scratch.
Set annealing temperature range
Optima 96 has six independent heating/cool modules for PCR optimization and excellent gradient temperature accuracy (+0.1°C). A 5°C differential can be set between each of the 6 modules allowing for ultimate PCR optimization and a 25°C span for analyzing performance temperatures in a single run.
Start & Monitor PCR reaction
Monitor results displayed on Optima 96’s new 7-inch LCD Touchscreen panel, making it easy to set up and analyze for performance. Quickly modify and save programs and watch your gels come to life. Designed to be used in GMP and non-GMP PD laboratories or for routine PCR workflows.
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Specification
| Model | PR122 |
| Sample Capacity | 0.2mL PCR Tubes，0.2mL 8-tube Strips，0.2mL 96-well PCR Plate (non-skirted/semi-skirted) |
| PCR reaction volume | 10-100 µl |
| Heating lid temperature | 30-110°C |
| Max. block heating rate | 5°C/s |
| Max. block cooling rate | 4°C/s |
| Sample temperature range | 4°C-100°C |
| Temperature uniformity | <0.5°C (20s after reaching 95°C ) |
| Temperature accuracy | ±0.1°C (35 °C - 99.9°C) |
| Technology(heating/cooling) | Semiconductor Peltier technology 6 independent blocks |
| Display resolution | 0.1°C |
| Maximum temperature difference between blocks | 25°C (max. 5°C between two adjacent blocks) |
| Difference between two adjacent blocks | 0.1°C-5.0°C |
| Gradient temperature accuracy | 9±0.1°C (35°C - 99.9°C) |
| Gradient temperature accuracy | <0.5°C (20s after reaching 95°C) |
| Display | 7" LCD touch screenreal-time display of PCR process |
| Max. number of steps | No limit |
| Time increment/decrement | 1-120s for long Range PCR experiment |
| Temperature increment/decrement | 0.1°C-10.0°C for Touchdown PCR |
| Maximum number of cycles | Unlimited |
Accurate & Sensitive
Optima 96’s uses 6 separate thermoelectric modules resulting in high level of accuracy and sensitivity in detecting and amplifying target DNA sequences, even in low concentrations.
Quicker heating and cooling
Equipped with one of the fasting heating and cooling rates in the industry, with a maximum heating rate of >5°C/s and a maximum cooling rate of >4°C/s. This feature ensures better nucleotide pairing performance and reduces the overall time required for PCR reactions, making Optima 96 ideal for high-throughput applications.
Versatile Components
Optima 96’s segmented heating lid design automatically adjusts to different types of PCR tubes, strips, and plates, making it compatible with a wide range of PCR applications. In addition, the device offers advanced features such as Touchdown (TD) PCR Mode and Long-Range PCR Modes, further enhancing versatility and customization of the PCR process.
Accurate results & easier data storage
The six separate imported thermoelectric modules ensures accurate results for PCR optimization and performance on the edges of 96 well plates. Including a front-facing USB interface for easy program import and export, software updates, and multiple accounts and access rights, Optima 96 makes data storage and analysis easier than ever.
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